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帶有光保護的醌甲基化物（QM）小分子化學交聯劑。使用這些交聯劑，可以通過遠紫外光（365 nm）誘導從而特異性的釋放活化的醌甲基化物進行蛋白質-蛋白質以及蛋白質-核酸之間的交聯。僅能夠使得蛋白交聯獲得時間分辨率，引入的強活性的醌甲基化物可與許多鄰近的親核性的殘基，如 His、Lys、Tyr 等側鏈反應而產生蛋白交聯。并且這些交聯劑不僅使適用單個蛋白的交聯，也可以在細胞裂解液以及在活體細胞中誘導蛋白-蛋白發生化學交聯。

合成一端帶有醌甲基化物前體，另一端帶有NHS酯基的小分子化學交聯劑（NHQM）。通過對14-3-3 目標蛋白紫外光照射與否以及加不加NHQM交聯劑處理，發現只有在加入NHQM交聯劑并且紫外光照射下才會誘導產生14-3-3二聚體。并且這種二聚體的產生可以很好的通過光控實時的產生。進一步證明了NHQM交聯劑的其中一個實用性-可以快速鑒別蛋白聚合情況，并且利用化學交聯質在14-3-3蛋白上與醌甲基化物反應的親和性殘基。

接著，測試了NHQM在細胞內的交聯情況，發現細胞體內交聯效率較弱，這可能與短時間內NHQM穿膜性和溶解性差有關，而增長穿膜時間NHS會快速水解從而使交聯失效

齊岳供應產品：

產品列表：

DHB

2,5-dihydroxybenzoic acid

CAS Number: 490-79-9

CHCA

α-Cyano-4-hydroxycinnamic acid

CAS Number: 28166-41-8

HABA

2-(4'-Hydroxybenzeneazo)benzoic acid

CAS Number: 1634-82-8

Sinapinic Acid

CAS Number: 530-59-6

THAP

2',4',6'-Trihydroxyacetophenone monohydrate

CAS Number: 480-66-0

DHAP

2',4',-Dihydroxyacetophenone

CAS Number: 89-84-9

MBT

MBT MALDI matrix, 2-Mercaptobenzothiazole

CAS Number: 149-30-4

色譜相關試劑

產品列表：

LCMS Grade Solvents：

LCMS Acetonitrile CAS Number: 75-05-8

LCMS Acetonitrile with 0.1% Acetic Acid

LCMS Acetonitrile with 0.1% Formic Acid

LCMS Acetonitrile with 0.1% TFA

LCMS Acetonitrile with 0.1% Formic Acid and 0.01% TFA

LCMS Water with 0.1% Acetic Acid

LCMS Water with 0.1% Formic Acid

LCMS Methanol CAS Number: 67-56-1

LCMS Water CAS Number: 7732-18-5

HPLC Grade Solvents：

HPLC Acetone CAS Number: 67-64-1

HPLC Acetonitrile CAS Number: 75-05-8

HPLC Acetonitrile with 0.1% TFA

HPLC Cyclohexane CAS Number: 110-82-7

HPLC Dimethyl sulfoxide CAS Number: 67-68-5

HPLC Methanol CAS Number: 67-56-1

HPLC Water CAS Number: 7732-18-5

Acetic Acid Ampules (Proteomics Grade) CAS Number: 64-19-7

Formic Acid Ampules (Proteomics Grade) CAS Number: 64-18-6

TFA Ampules (Proteomics Grade) CAS Number: 76-05-1

HFBA Ampules (Proteomics Grade) CAS Number: 375-22-4

Formic Acid + TFA Ampules (Proteomics Grade)

LCMS Mobile Phases Sampler Pack

Additives & Ion Pairing：

Proteochem蛋白交聯試劑，進口蛋白交聯試劑

Protein crosslinkers （蛋白交聯相關試劑）：

Heterobifunctional Crosslinkers異型雙功能交聯劑：

SMCC Crosslinker

Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate

CAS Number: 64987-85-5

Sulfo-SMCC Crosslinker

Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate

CAS Number: 92921-24-9

Sulfo-SANPAH Crosslinker

N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate

CAS Number: 102568-43-4

SMPH Crosslinker

Succinimidyl-6-[?-maleimidopropionamido]hexanoate

PDPH Crosslinker

3-[2-Pyridyldithio]propionyl hydrazide) is a cleavable, carbohydrate reactive

MBS Crosslinker

m-Maleimidobenzoyl-N-hydroxysuccinimide ester

CAS Number: 58626-38-3

Sulfo-MBS Crosslinker

m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester

SPDP Crosslinker

N-Succinimidyl 3-[2-pyridyldithio]-propionate

CAS Number: 68181-17-9

LC-SPDP Crosslinker

Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate

Sulfo-LC-SPDP Crosslinker

Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate

SIA Crosslinker

N-Succinimidyl iodoacetate

CAS Number: 39028-27-8

SBA Crosslinker

N-Succinimidyl bromoacetate; SIB crosslinker

CAS Number: 42014-51-7

SMPB Crosslinker

N-Succinimidyl 4-[4-maleimidophenyl]butyrate

CAS Number: 79886-55-8

BMPS Crosslinker

N-(?-Maleimidopropyloxy)succinimide ester

CAS Number: 55750-62-4

EMCS Crosslinker

N-[e-Maleimidocaproyloxy]succinimide ester

CAS Number: 55750-63-5

GMBS Crosslinker

N-[Gamma-Maleimidobutyryloxy] Succinimide

CAS Number: 80307-12-6

ANB-NOS Crosslinker

N-5-Azido-2-nitrobenzoyloxysuccinimide

CAS Number: 60117-35-3

Homobifunctional Crosslinkers同型雙功能交聯劑：

BS3 Crosslinker

Bis[sulfosuccinimidyl] suberate

CAS Number: 82436-77-9

DSS Crosslinker

Disuccinimidyl suberate N-hydroxysuccinimide ester

CAS Number: 68528-80-3

BS2G Crosslinker

BS2G; Bis[Sulfosuccinimidyl] glutarate

DSG Crosslinker

Disuccinimidyl glutarate; Di(N-succinimidyl

CAS Number: 79642-50-5

DSP Crosslinker

3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester)

CAS Number: 57757-57-0

DTSSP Crosslinker

3,3′-Dithiobis[sulfosuccinimidylpropionate

CAS Number: 81069-02-5

Sulfo-EGS Crosslinker

glycolbis(sulfosuccinimidylsuccinate)

CAS Number: 167410-92-6

EGS Crosslinker

Ethylene glycolbis(succinimidylsuccinate)

CAS Number: 70539-42-3

DST Crosslinker

CAS Number: 62069-75-4

Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS：

EDC-HCl

1- Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride

CAS Number: 25952-53-8

Sulfo-NHS

N-hydroxysulfosuccinimide Hydroxy-2,5-dioxopyrrolidine-3-sulfonicacid

CAS Number: 106627-54-7

進口蛋白質交聯試劑列表，Sulfo-LC-SPDP

Protein crosslinkers （蛋白交聯相關試劑）：

Heterobifunctional Crosslinkers異型雙功能交聯劑：

SMCC Crosslinker CAS Number: 64987-85-5

Sulfo-SMCC Crosslinker CAS Number: 92921-24-9

Sulfo-SANPAH Crosslinker CAS Number: 102568-43-4

SMPH Crosslinker

PDPH Crosslinker

MBS Crosslinker CAS Number: 58626-38-3

Sulfo-MBS Crosslinker

SPDP Crosslinker CAS Number: 68181-17-9

LC-SPDP Crosslinker

Sulfo-LC-SPDP Crosslinker

SIA Crosslinker CAS Number: 39028-27-8

SBA Crosslinker CAS Number: 42014-51-7

SMPB Crosslinker CAS Number: 79886-55-8

BMPS Crosslinker CAS Number: 55750-62-4

EMCS Crosslinker CAS Number: 55750-63-5

GMBS Crosslinker CAS Number: 80307-12-6

ANB-NOS Crosslinker CAS Number: 60117-35-3

Homobifunctional Crosslinkers同型雙功能交聯劑：

BS3 Crosslinker CAS Number: 82436-77-9

DSS Crosslinker CAS Number: 68528-80-3

BS2G Crosslinker

DSG Crosslinker CAS Number: 79642-50-5

DSP Crosslinker CAS Number: 57757-57-0

DTSSP Crosslinker CAS Number: 81069-02-5

Sulfo-EGS Crosslinker CAS Number: 167410-92-6

EGS Crosslinker CAS Number: 70539-42-3

DST Crosslinker CAS Number: 62069-75-4

Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS：

EDC-HCl CAS Number: 25952-53-8

Sulfo-NHS CAS Number: 106627-54-7

Deuterated Crosslinker BS3-d4

Deuterated Crosslinker BS2G-d4

Deuterated Crosslinker DSS-d4

上述產品齊岳生物均可供應，僅用于科研！

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